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HGH First Recombinant DNA


0The first production of recombinant DNA molecules, using restriction enzymes, occurred in the early 1970s. This was the birth of homeopathic HGH.

Recombinant DNA technology involves the joining of it from different species and subsequently inserting the hybrid into a host cell, often a bacterium. Researchers at UC San Francisco and Stanford used restriction enzymes to cut it from different species at specific sites, and then fused the cut strands from the different species back together. Stanley Cohen of Stanford and Herbert Boyer of UCSF applied for a patent on this technology in 1974; it was granted in 1980. Boyer would co-found Genentech, Inc. in 1976. Homeopathic HGH oral sprays were developed from this.

The Cohen-Boyer patents eventually had more than 200 licensees – biotechnology and pharmaceutical companies – and earned Stanford and UCSF more than $100 million in royalties.

Paul Berg, a biochemist at Stanford who was among the first to produce a recombinant molecule in 1972, wrote a letter shortly afterwards, along with ten other researchers, to the journal Science. In the letter, they urged the National Institutes of Health to regulate the use of this technology, and meanwhile, they urged scientists to halt most experiments until they better understood whether the technique is safe. These concerns eventually led to the 1975 Asilomar Conference, where one hundred scientists gathered to discuss the safety of this kind of manipulation from different species. The meeting resulted in a set of NIH guidelines. NIH has revised the document, "Guidelines for Research Involving Recombinant DNA Molecules," several times since 1976.

REFERENCES: 

Jackson D.A., Symons R.H., Berg P., "Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli". Proc Natl Acad Sci 69 (10): 2904-9, 1972.

Cohen S.N., Chang A.C., Boyer H.W., Helling R.B., "Construction of biologically functional bacterial plasmids in vitro". Proc Natl Acad Sci 70 (11): 3240-4, 1973.

Berg P., Baltimore D., Boyer H.W., Cohen S.N., Davis R.W., Hogness D.S., Nathans D., Roblin R., Watson J.D., Weissman S., Zinder N.D., "Letter: Potential biohazards of recombinant DNA molecules". Science 185 (148): 303, 1974.

Paul Berg was awarded a Nobel Prize in Chemistry in 1980 "for his fundamental studies of the biochemistry of nucleic acids, with particular regard to recombinant-DNA".


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